Presentation Title

Separation and Conductivity Detection of Inorganic Ions Using Capillary Electrophoresis

Presentation Type

Poster

School

School of Sciences and Social Sciences

Discipline

Chemistry

Mentor

James Kraly

Abstract

The goal of this project is to implement a method for the separation and measurement of inorganic ions using an analytical technique called Capillary Electrophoresis. Environmental and biological research often requires quantifying concentrations of simple ions in aqueous solution mixtures. Capillary electrophoresis separates mixtures within a thin glass capillary using a high voltage and measures ions individually. Concentrations of ions in water samples will be determined using standard chemicals, calibration curves, and a specialized conductivity detector. The conductivity detector measures separated ions where concentration is related to conductivity. Initial chemical conditions for separation and programmed instrumental detection parameters will be applied from the chemical literature, and optimized for our system. Our goal is to produce two procedures that allow the rapid and sensitive separation of positively charged ions, and negatively charged ions, respectively. Example separations will be presented to demonstrate instrumental performance and to determine analytical figures of merit.

Grant Funded

1

Type of Grant

Student Grant

Grant Name

Undergraduate Research and Creative Projects

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Separation and Conductivity Detection of Inorganic Ions Using Capillary Electrophoresis

The goal of this project is to implement a method for the separation and measurement of inorganic ions using an analytical technique called Capillary Electrophoresis. Environmental and biological research often requires quantifying concentrations of simple ions in aqueous solution mixtures. Capillary electrophoresis separates mixtures within a thin glass capillary using a high voltage and measures ions individually. Concentrations of ions in water samples will be determined using standard chemicals, calibration curves, and a specialized conductivity detector. The conductivity detector measures separated ions where concentration is related to conductivity. Initial chemical conditions for separation and programmed instrumental detection parameters will be applied from the chemical literature, and optimized for our system. Our goal is to produce two procedures that allow the rapid and sensitive separation of positively charged ions, and negatively charged ions, respectively. Example separations will be presented to demonstrate instrumental performance and to determine analytical figures of merit.