Presentation Title

Using Chemistry to Find a New Way to Treat Cholera

Presentation Type

Poster

School

School of Sciences and Social Sciences

Discipline

Chemistry

Mentor

Paul Baures

Abstract

The objective of this research is to create a potential inhibitor of Type IV prepilin peptidase to prevent the autoagglutination, or clumping of bacterial cells, by preventing pili formation of V. cholerae. Substrates must be a particular size or shape to be recognized by an enzyme, much like how two puzzle pieces fit together. Three different potential enzyme inhibitor designs were selected based on their structural similarity to the natural substrate. Up to this point the focus of our research remains on optimizing small scale reactions to obtain high yields and purity. We have employed microwave instrumentation to both shorten the reaction and improve our yields. Once the final products are synthesized, purified, and characterized, they will be tested for their ability to prevent the autoagglutination by adding the compounds to the solution used to grow the bacteria. Since the proposed structure mimics the natural substrate, it will prove to be beneficial because the body is less likely to reject a familiar substance, allowing the treatment to work.

Grant Funded

1

Grant Name

NH-INBRE

Share

COinS
 

Using Chemistry to Find a New Way to Treat Cholera

The objective of this research is to create a potential inhibitor of Type IV prepilin peptidase to prevent the autoagglutination, or clumping of bacterial cells, by preventing pili formation of V. cholerae. Substrates must be a particular size or shape to be recognized by an enzyme, much like how two puzzle pieces fit together. Three different potential enzyme inhibitor designs were selected based on their structural similarity to the natural substrate. Up to this point the focus of our research remains on optimizing small scale reactions to obtain high yields and purity. We have employed microwave instrumentation to both shorten the reaction and improve our yields. Once the final products are synthesized, purified, and characterized, they will be tested for their ability to prevent the autoagglutination by adding the compounds to the solution used to grow the bacteria. Since the proposed structure mimics the natural substrate, it will prove to be beneficial because the body is less likely to reject a familiar substance, allowing the treatment to work.